This project automates DNA transformation and gene cloning protocols on the Opentrons OT-2 platform, improving throughput and reproducibility for routine lab workflows.
Automated DNA Transformation and Gene Cloning (Opentrons OT-2)
Automation workflows for DNA transformation and gene cloning on the Opentrons OT-2 platform.
Key Reminders
- Describe the end-to-end protocol automation and error handling.
- Discuss calibration and volume accuracy considerations.
- Highlight throughput and reproducibility gains.
Technologies
Problem and Scope
Automated DNA transformation and gene cloning protocols to improve reproducibility and reduce manual pipetting overhead.
System Architecture
Protocols were implemented as modular steps for reagent preparation, transformation, and incubation, with structured logging for traceability.
python code
Code example forthcoming
Opentrons protocol step definition.
Hardware and Calibration
Calibration routines ensured accurate deck positioning and volume transfers across repeated runs.
Testing and Validation
Validation emphasized repeatability, contamination avoidance, and run-time reliability.
Demo and Next Steps
Future work includes expanded protocol libraries and integration with lab information systems.